AlphaSTEM™ Culture System
The AlphaSTEM™ Culture System uses a newly discovered, naturally occurring growth factor that is expressed in the earliest days of embryogenesis to fully support the naïve state in human stem cells. Naïve stem cells have several advantages over current stem cells, called ‘primed’ state cells, which are important for the future of stem cell research and regenerative medicine. Naïve stem cells do not yet have DNA methylation marks that commit the cells to certain developmental decisions. Additionally, naïve stem cells have a much higher cloning efficiency than primed state cells.
System Benefits
- One single naturally occurring growth factor
- Enzymatically passage as single cells
- No clonal restriction; every iPS clone able to form
cardiomyocytes, hepatocytes, neural progenitors
- Superior differentiation with improved yield,
cell quality, and functionality
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- Feeder-free iPSC generation that is simpler and orders of
magnitude more efficient than in FGF media
- Expansion that is faster, scalable and can be completely
automated since it eliminates spontaneous differentiation
- Karyotype stable > 70 passages
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The AlphaSTEM™ Culture System is a superior alternative to FGF-based stem cell culture
AlphaSTEM® Naïve hPSC Medium
Stem Cell Culture Medium | 500ml AlphaSTEM® Naïve hPSC Medium is a serum-free and feeder-free medium formulated for the generation and expansion of naïve pluripotent stem cells. Utilizing a naturally occurring growth factor, NME7 AB , that is expressed in the earliest days of embryogenesis, AlphaSTEM® Naïve hPSC Medium fully supports the...
add to cartAlphaSTEM® Culture Substrate
Cell Culture Surface Coating | 360ul
AlphaSTEM® Culture Substrate is a mouse monoclonal antibody that provides a defined surface for feeder-free generation and expansion of naive pluripotent stem cells. AlphaSTEM® Culture Substrate binds to the MUC1* growth factor receptor that is expressed on all pluripotent human stem cells. When used with AlphaSTEM®...
add to cartAlphaSTEM® Differentiation Inducer
Synthetic peptide | 3 x 12ul AlphaSTEM® Differentiation Inducer is a synthetic peptide that competitively inhibits the activity of NME7 AB, the naturally occurring growth factor in AlphaSTEM® Naive hPSC Medium. AlphaSTEM® Differentiation Inducer is added one time when differentiation is initiated and can be added along with any other reagents used...
add to cartAlphaSTEM® Naïve iPSC Line, Female
Naïve Stem Cell Line | 1M AlphaSTEM® Naïve iPSC Line, Female is a zero-footprint, viral-integration-free human induced pluripotent stem cell (iPSC) line derived from peripheral blood mononuclear cells (PBMCs). These cells have the gene expression signature of naïve state cells and have a stable karyotype for over 50 passages when cultured in the...
add to cartAlphaSTEM® Naïve iPSC Line, Male
Naïve Stem Cell Line | 1M AlphaSTEM® Naïve iPSC Line, Male is a zero-footprint, viral-integration-free human induced pluripotent stem cell (iPSC) line derived from newborn foreskin fibroblasts. These cells have the gene expression signature of naïve state cells and have a stable karyotype for over 50 passages when cultured in the...
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